FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing.

FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing.

Blog Article

Massively parallel zwo off axis guider sequencing of cell-free, maternal plasma DNA was recently demonstrated to be a safe and effective screening method for fetal chromosomal aneuploidies.Here, we report an improved sequencing method achieving significantly increased throughput and decreased cost by replacing laborious sequencing library preparation steps b-0665-cr-bstr with PCR employing a single primer pair designed to amplify a discrete subset of repeated regions.Using this approach, samples containing as little as 4% trisomy 21 DNA could be readily distinguished from euploid samples.